Effects of hS100A2 and hS100A6 on Human Colorectal Cancer Cell ...

[Abstract]

BACKGROUNDS AND OBJECTIVES:The pathogenesis and development of tumor is a complex biologic process that involves various factors and numerous genes, including many signal molecules. Therefore, it is very important to study the effects and the mechanisms of tumor-related signal molecules, and the reciprocal relationships among these signal molecules for elucidating the mechanisms of the pathogenesis and development of tumor.The S100 proteins are small acidic signaling proteins (9?13 kDa) that are found exclusively in vertebrates.With at least 25 members found to date in humans, the S100 proteins constitute the largest subfamily of the EF-hand proteins. S100 proteins are proposed to have intracellular and extracellular roles in the regulation of many diverse processes such as protein phosphorylation, cell growth and motility, cell-cycle regulation, transcription, differentiation and cell survival. 21 members (S100A1?S100A18, trichohylin, filaggrin and repetin) of these proteins have genes clustered at chromosome locus 1q21, which has low stability. A potential role for S100 proteins in neoplasia stems from these activities and from the observation that several S100 proteins (including S100A2 and S100A6) have altered levels of expression in different stages and types of cancers. But the precise roles and the importance of S100 proteins in the development and promotion of cancer are poorly understood.The S100A2 is often downregulated in cancers, including melanoma, breast cancer, prostate cancer, lung tumors, so on. Furthermore, it has been indicated as a potential tumor suppressor by differential hybridization. But there are still opposite reports.The S100A6 protein was first purified from Ehrlich ascites tumor by Kuznicki and Filipek. The overexpression of S100A6 has been reported in several types of cancers, such as epithelial carcinomas (colon cancer, hepatocellular cancer, cholangiocarcinoma, pancreatic cancer and malignant melanoma), osteosarcoma, ovarian cancer, ras tansformed NIH3T3 cells and SV40 transformed mouse fibroblast. But the exact effects of S100A6 in cancers remain unknown.In order to elucidate the effects and the mechanisms of S100A2 and S100A6 on human tumors and to find some new markers for tumor diagnosis and prognosis, and some new targets for tumor therapy, the effects of S100A2 and S100A6 on cell proliferation, cell cycle, apoptosis and migration of human colorectal cancer cell line HCT116 and human osteosarcoma cell line MG63 were investigated; Furthermore, the effects of S100A2 and S100A6 on the level and distribution of?-catenin in MG63 cells were studied.METHODS1. The preparation and identification of recombinant hS100A2 and hS100A6 protein: pGST-Moluc-hS100A2 and pGST-Moluc-hS100A6 were digested by restriction endonucleases, Xho?and EcoR, and then analyzed by agarose gel electrophoresis. E Coli. (BL21) was transformed with the two plasmids respectively and the expressions of GST-hS100A2, and GST-hS100A6 were induced by IPTG. Recombinant hS100A2 and hS100A6 proteins were purified by Glutathione Sepharose 4B beads and identified by SDS-PAGE/ Western Blot. Their concentrations were determined by Bradford.2. The recombinant adenoviruse of S100A6 (AdS100A6) was amplified in human embryonic kidney cell 293(HEK293 cell); hS100A6 protein in the cell lysate of HCT116 infected by AdS100A6 was detected by SDS-PAGE/ Western Blot.3. HCT116 cells and MG63 cells were treated with GST, GST-hS100A2, GST-hS100A6, AdGFP, and AdS100A6, respectively. The final concentration of all proteins was 40?g/ml. Cell proliferation was measured by MTT method and trypan blue stain/cell counting; And cell cycle(PI stain) and cell apoptosis(annexin V-PE and 7AAD stain) were detected by flow cytometry; the cell migration ability was determined by transwell chambers; the changes of?-catenin expression were observed under laser scanning confocal microscope (LSCM) after immunofluorescence stain.RESULTS1. The two plasmids were digested into two fragments, about 300bp and 9kb, respectively. The results were consistent with their maps of restriction endonucleases. hS100A2 and hS100A6 were found in the two purified recombinant proteins ( SDS-PAGE/ Western blot), respectively. The yields of recombinant hS100A2 and hS100A6 were 5mg/L and 3mg/L, respectively (Bradford).2. hS100A6 protein was found in the cell lysate of HCT116 infected by AdS100A6( SDS-PAGE/ Western blot).3. The effects of exogenous S100A2 and S100A6 on cell proliferation of HCT116 cells and MG63 cells.The proliferation of HCT116 cell was inhibited from the 4th day and 5th day after respectively treated with recombinant hS100A2 and hS100A6 proteins (P0.05) of those in AdGFP group, respectively. The effects of hS100A2 and hS100A6 on MG63 cell cycle were similar to theirs on HCT116 cell cycle.These results indicated that both S100A2 and S100A6 might block cell cycles of HCT116 cells and MG63 cells by increasing G0~G1 stage cells and decreasing S stage cells. Recombinant hS100A6 protein and AdS100A6 had similar activities on cell cycles of the two cell lines.5 The effects of exogenous S100A2 and S100A6 on cell apoptosis of HCT116 cells and MG63 cellsAfter HCT116 cells were cultured with recombinant hS100A2 and hS100A6 protein for 48 hours, the apoptosis cell numbers were 2.3 times and 2.0 times as those of GST control groups, respectively (P0.05) and showed a tendency of increase.These results indicated that both S100A2 and S100A6 could promote the apoptosis of HCT116 cells and MG63 cells.6. The effects of exogenous S100A2 and S100A6 on cell migration of HCT 116 cells and MG63 cellsCompared with their own controls, the cell numbers of trans-membrane of HCT116 cells and MG63 cells after treated with recombinant hS100A2 protein for 60 hours decreased by 75 % and 83%, respectively(P

Title: Effects of hS100A2 and hS100A6 on Human Colorectal Cancer Cell Line HCT116 and Human Osteosarcoma Cell Line MG63

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Source: http://www.tumorres.com/pituitary-tumor/34457.htm

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NJ gov strongly defends lowering flags for Houston (AP)

NEWARK, N.J. ? Gov. Chris Christie has strongly defended his decision to have flags flown at half-staff Saturday for Whitney Houston despite receiving emails and other messages disparaging the singer and criticizing him.

The Republican governor said Wednesday that he rejects complaints that Houston "forfeited the good things that she did" because of her struggles with substance abuse.

"What I would say to everybody is there but for the grace of God go I," he said.

Christie ordered flags flown at half-staff at state government buildings Saturday, the day funeral services are held for Houston at the Newark church she sang at as a child.

Twitter was abuzz Wednesday with reaction to the decision by Christie, whose home is in Mendham, a wealthy town where Houston also had lived.

In online postings, there were two main arguments against the honor for the Grammy Award winner who died over the weekend in California at age 48: One was that it should be reserved for members of the military, first responders and elected officials. The other was that it's wrong to honor a drug addict.

Heather Clause, a Richmond, Va.-based blogger who writes about teen moms and was tweeting critical comments, said in a telephone interview that she was appalled by the planned flag-lowering.

"It's just such a bad example for people," said 23-year-old Clause. She said the decision was like saying if someone sings well, drug use doesn't matter and "you can still be an idol."

In upstate New York, Rebecca Eppelmann, a newspaper copy editor, also tweeted her disgust at the Houston honor, then discussed her views.

"It should be for major events," she said. "It's horrible that she passed away. It's not something that should warrant this."

Christie said he was not saying that Houston, who was born in Newark and was raised in nearby East Orange, is a role model. Instead, he said, Houston deserves the honor because of her huge cultural impact and as "a daughter of New Jersey."

"I am disturbed by people who believe that because her ultimate demise ? and we don't know what is the cause of her death yet ? but because of her history of substance abuse that somehow she's forfeited the good things that she did in her life," said the governor during a briefing in northern New Jersey. "I just reject that on a human level."

The cause of Houston's death at a Beverly Hills hotel has not been determined, and the results of toxicology tests are pending. Investigators found several bottles of prescription medication in the hotel room where she died Saturday, but authorities said they weren't an unusually large number.

The governor noted he has ordered flags flown at half-staff for all 31 fallen New Jersey soldiers and every fallen police officer during his time in office.

He also ordered flags lowered last year for Clarence Clemons, the saxophonist for Bruce Springsteen's E Street Band.

In Newark, residents were irritated to hear of criticism of the governor's decision to honor Houston, who acknowledged her drug abuse in interviews.

Resident Anna Simpson said she admires Christie for honoring Houston because "if it were Bruce Springsteen or Jon Bon Jovi, nobody would bat an eye."

Simpson was at New Hope Baptist Church Wednesday, where Houston's funeral will be held, to pay her respects on her way home from her airport job.

"She never forgot where she came from," Simpson said. "She was real. We would see her and be like, `She's one of ours,' and she always made us proud, no matter what happened."

After Houston became famous she continued to make regular trips to the public school she attended in East Orange and to which her family has directed donations be sent in lieu of flowers.

___

Associated Press writer Geoff Mulvihill in Haddonfield, N.J., contributed to this report.

Source: http://us.rd.yahoo.com/dailynews/rss/celebrity/*http%3A//news.yahoo.com/s/ap/20120216/ap_en_mu/us_whitney_houston_flags

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Janice Voss, veteran of 5 space shuttle flights, dies at 55

Astronaut Janice Voss, a veteran of five spaceflights and a former science director for a NASA exoplanet-hunting spacecraft, has died after a battle with cancer. She was 55.

"Just got the very sad news that U.S. astronaut Janice Voss passed away last night," the Association of Space Explorers, an international organization representing more than 350 individuals who have flown in space, wrote on Facebook. "Our thoughts go out to her family and friends."

NASA confirmed Voss' death in a statement issued on Tuesday, saying she had passed away overnight.

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Chosen by NASA for the astronaut corps in January 1990, Voss served as mission specialist on five space shuttle missions, including the only repeat flight in the shuttle program's 30-year history. She flew with the first commercial laboratory, rendezvoused with Russia's Mir space station and helped create the most complete digital topographic map of the Earth.

Five-time shuttle flier
Voss launched on her first and final missions aboard the shuttle Endeavour. As a member of the STS-57 crew in June 1993, she helped conduct biomedical and material science experiments in the first commercially-developed Spacehab module, a pressurized laboratory mounted in the orbiter's payload bay that more than doubled the work area for astronaut-tended activities.

In February 2000 Voss again launched on Endeavour, this time for NASA's Shuttle Radar Topography Mission. After deploying a nearly 200-foot mast, Voss and her crewmates worked around the clock in two shifts to map more than 47 million square-miles of the Earth's land surface. [ Shuttle's Best Science Missions ]

Voss' second flight to space marked the first time a space shuttle came within the vicinity of Russia's space station Mir. Flying on the shuttle Discovery, Voss and her STS-63 crewmates ? including Eileen Collins, the first woman to pilot a U.S. spacecraft ? rendezvoused with the Russian outpost to verify flight techniques, communications, and navigation and sensor aids. The February 1995 "Near-Mir" mission set the stage for the first shuttle-Mir docking later that year.

Voss' two other spaceflights, STS-83 and STS-94, were the only time in the shuttle program's history that an entire crew was launched twice to achieve the same mission. The crew's first attempt began with a liftoff on Columbia on April 4, 1997. Three days into the mission however, a problem with one of the orbiter's three power-generating fuel cells resulted in the flight being cut short and the crew members returning to Earth.

Three months later with Columbia back in working order, Voss and her six STS-83 crewmates launched again, this time as the STS-94 crew. During the successful 15-day flight, Voss and her fellow fliers worked inside a European Spacelab module, conducting experiments as part of the Microgravity Science Laboratory (MSL) mission.

In total, Voss logged over 49 days in space, traveling 18.8 million miles while circling the Earth 779 times. Her five missions tied her with the record for the most spaceflights by a woman.

Four years after returning to Earth for a final time, Voss transferred from Johnson Space Center in Houston, Texas, to NASA's Ames Research Center at Moffett Field, Calif., where she headed the science program for the agency's Kepler space observatory. Designed to search for Earth size planets orbiting distant stars, Kepler was launched in March 2009 and to date has confirmed 61 exoplanets and identified more than 2,000 planetary candidates.

Voss left Ames in 2007 and most recently served as the payload lead in the astronaut office's space station branch at the Johnson Space Center.

"As the payload commander of two space shuttle missions, Janice was responsible for paving the way for experiments that we now perform on a daily basis on the International Space Station," chief astronaut Peggy Whitson said in the NASA statement.

"By improving the way scientists are able to analyze their data, and establishing the experimental methods and hardware necessary to perform these unique experiments, Janice and her crew ensured that our space station would be the site of discoveries that we haven't even imagined."

"During the last few years, Janice continued to lead our office's efforts to provide the best possible procedures to crews operating experiments on the station today," she said. "Even more than Janice's professional contributions, we will miss her positive outlook on the world and her determination to make all things better."

Path to space
A native of Rockford, Ill., Voss received her bachelor of science in engineering science from Purdue University in 1975, a master of science degree in electrical engineering and a doctorate in aeronautics and astronautics from the Massachusetts Institute of Technology in 1977 and 1987, respectively.

Voss' first work with NASA was during her undergraduate studies at Purdue. A member of Johnson Space Center's co-op program, she worked on computer simulations in the engineering and development directorate in the years leading up to the start of the shuttle-era. Voss returned to the Johnson in 1977 for a year, working as a crew trainer teaching entry guidance and navigation.

Before becoming an astronaut, Voss worked at the Orbital Sciences Corporation, supporting mission integration and flight operations for the Transfer Orbit Stage that launched the Advanced Communications Technology Satellite from the space shuttle in September 1993, and NASA's ill-fated Mars Observer from a Titan rocket in 1992.

A multiple recipient of NASA's Space Flight Medal, Voss donated her personal papers documenting her spaceflight career to Purdue Libraries' division of archives and special collections in 2009.

"Knowing that someone else got from here to there brightened many of my days at Purdue," Voss said at the time, referring to the university's earlier astronaut alums. "Maybe my papers will help someone else feel that they aren't that different from me."

"If I can do it, then so can they," Voss said.

Follow collectSPACE on Facebook and Twitter @ collectSPACE and editor Robert Pearlman @ robertpearlman. Copyright 2011 collectSPACE.com. All rights reserved.

? 2012 Space.com. All rights reserved. More from Space.com.

Source: http://www.msnbc.msn.com/id/46302650/ns/technology_and_science-space/

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Sports Recreation: Minimize Stress and Have Fun | Where To ...

Therefore, everyone needs some time to unwind and have some entertainment. Our modern way of
living also tends to be inactive, and the meals most people eat are often not very healthy. Exercise is a
very important component in restarting us. For these reasons, sports recreation pursuits are some
of the best fun we can choose. There are wide ranging options for hobbies to offer sports
recreation. You can even want to learn some new leisurely sports skills.

Training systems to learn new sports recreation skills can be found online and in much bigger cities. You can understand
and participate in workforce sports through the community Y and other businesses. Team sports recreation
functions you might like to participate in comprise softball, baseball, hockey, and sometimes
soccer designed for younger people. Another squad sport you might appreciate is bowling. Regardless of the
skill level you can find your bowling team to have fun with.

Particular person sports recreation pursuits you can learn and participate in include golf, tennis games, running,
aerobic party, gymnastics, and horseback riding. Don?t overlook bicycling and also weight training too.
Many of these sports can be figured out at the local Gym as well. For some of these, you need to have
a friend to relax and play against or share the exerience with. Countless runners, for instance, prefer to run with a
colleague. And games like football and handball really need cooperation for best results.

If you would rather, you can learn concerning sports recreation expertise online. You can find training to teach
almost any outdoor activity or activity you can think of including underwater handbags, fly-fishing, and
para-gliding in the Alps! Needless to say, unless you can get some hands-on knowledge you?ll only have
any theories of the outdoor activity. Still, online courses are a way to get a taste of sports
and recreational activities you might someday would like to try out.

A really enjoyment sports recreation activity you might get a chance to participate in is ping pong. Ping pong, or
table tennis as it is sometimes called, is played on a table with rubber coated paddles and
extremely compact plastic balls.
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You ought to hit the ball best honeymoon destinations with your paddle so it strikes the meal table and clears the online before your
adversary strikes it and bounces it back to you. One way to become more adept at table tennis and
increase your probability of winning is to oftentimes hit the baseball harder than sometimes. This
keeps your attacker guessing what you will undertake next.

Sports adventure activities are a great way to obtain exercise and relieve stress. They are also a lot
associated with fun. dresses to wear to a wedding Whether with close friends or alone, everyone needs a favorite sports recreation to take part in.

Source: http://www.wheretosubmitarticles.in/sports-recreation-minimize-stress-and-have-fun/

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Judge allows 'Sister Wives' suit to proceed

SALT LAKE CITY (AP) ? A federal judge has ruled there's sufficient evidence to allow a polygamous family made famous by a reality TV show to pursue a lawsuit challenging the constitutionality of Utah's bigamy law.

U.S. District Judge Clark Waddoups on Friday dismissed Utah's governor and attorney general from the case, but allowed the suit to proceed against Utah County Attorney Jeffrey Buhman, the Deseret News and Salt Lake Tribune report.

Buhman threatened to prosecute Kody Brown and his four wives ? Meri, Janelle, Christine and Robyn ? after the TLC show "Sister Wives" debuted in September 2010, but his office has not filed charges.

The family sued Buhman, Gov. Gary Herbert and Attorney General Mark Shurtleff in July 2011, claiming Utah's bigamy statute violates its constitutional rights to due process, equal protection, free exercise of religion, free speech and freedom of association.

Waddoups, in his 21-page ruling, wrote that he dismissed Herbert and Shurtleff from the case because Shurtleff assured the Browns that they wouldn't be prosecuted. Shurtleff has a policy of not prosecuting consenting adult polygamists as long as they're not committing other crimes.

But the judge wrote that Buhman conducted interviews with the news media that made it clear he intended to investigate and prosecute the Browns. The fact that no charges have been filed does not matter, he added.

"The entirety of actions by the Utah County prosecutors tend to show either an ill-conceived public-relations campaign to showboat their own authority and/or harass the Browns and the polygamist community at large, or to assure the public that they intended to carry out their public obligations and prosecute violations of the law," Waddoups wrote.

There's reason for the Browns to believe they could face prosecution in Utah County, the judge continued, and that could have a "chilling effect" on their ability to practice their First Amendment rights in the state.

But the Browns must show that there's a real and viable threat to their constitutional rights for the lawsuit to hold up in court, Waddoups wrote.

Brown moved his wives and 16 children from Lehi to the Las Vegas area in January 2001.

Buhman said he had not yet reviewed the ruling and was not prepared to comment.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/4e67281c3f754d0696fbfdee0f3f1469/Article_2012-02-04-Sister%20Wives-Lawsuit/id-11858ff52143488990ae1b19ef31981e

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Mad Catz Cyborg M.M.O. 7 gaming mouse hands-on

Mad Catz' Cyborg RAT series of adjustable computer mice has seen its fair share of minor revisions, and while wireless upgrades and whitewashed DPI tweaks have given consumers a hair of choice in transforming desktop pests, the differences between these devices has been modest, at best. Until now, at least. Enter the Cyborg M.M.O. 7, the outfit's latest addition to its aggressively styled line of gaming mice, minus the RAT moniker. Despite shedding the name of its predecessors, Mad Catz' latest point-and-clicker is as much of a RAT as the mice that came before it, but distinguishes itself with more buttons, more features and more color. Read on to see what's new, what's different and what should have stayed the same.

Gallery: Mad Catz Cyborg M.M.O. 7 gaming mouse hands-on

Continue reading Mad Catz Cyborg M.M.O. 7 gaming mouse hands-on

Mad Catz Cyborg M.M.O. 7 gaming mouse hands-on originally appeared on Engadget on Mon, 06 Feb 2012 10:00:00 EDT. Please see our terms for use of feeds.

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Networking tips offered to get next job ? Latin Business Today

By | Feb 5, 2012

UPLAND, Calif., Feb. 5 (UPI) -- It's purposeful networking, not simply schmoozing, that lands a person their next job, career coach Ford R. Myers says.

Myers' advice is timely, since Monday through Friday is International Networking Week, an initiative of BNI, an Upland, Calif., business networking and referral organization.

Myers, president of Career Potential LLC and author of "Get The Job You Want, Even When No One's Hiring," says job seekers can get hired through skillful networking, regardless of economic conditions.

"Networking is not about 'schmoozing,' or selling someone on the idea of hiring you," Myers advises in a release. "Rather, it's about purposefully and gracefully asking peers for help, advice, input and contacts -- and offering real value in return. Networking boosts job seekers' self-esteem and helps them connect people with opportunities -- especially when those opportunities are hard to come by."

Here are some tips from Myers to position yourself to get a job offer:

-- Schedule face-to-face meetings with your top tier contact list of people in the best position to help you.

-- Schedule phone meetings with other people who might lead you to key industry influencers.

-- Get at least 3-5 names from each person with whom you speak.

-- Be confident and purposeful.

-- Bring a list of questions and ask if it's OK to take notes.

-- Establish good rapport by getting the other person to talk about himself/herself.

-- Don't use the word "fired," be positive and focus on the future.

-- Don't make excuses or sound apologetic for your current career situation.

-- Look for opportunities to offer something of value in return for the other person's help and support.

-- Always send a "thank you" note immediately after your networking meeting.

Source: http://latinbusinesstoday.com/2012/02/networking-tips-offered-to-get-next-job/

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Integrating Business Unit Strategies Into a Synchronized Corporate ...

Once a business grows large enough to require separate divisions / units to support diverse lines of business, the challenges and complexity of strategic planning and strategy execution begin to grow as well. As the business portfolio expands, strategic planning models must adapt and change with the growing business for optimal results to be recognized.

Diversified businesses operate on models oriented to product and/or service lines. Often these are organized into lines of business that are managed by executives controlling their own P&L structures. Their measurements for success are tied to that particular line of business, meaning that their markets are unique and their cultures and organizational structures may not mirror those of the parent company or the other business units within the company. Likewise, the challenges they must confront to turn a profit and sustain growth are also not necessarily the same as their executive counterparts in other divisions of the company.

Bi-Directional Planning

Monolithic corporations comprised of multiple operating divisions need more granular approaches to strategy development, not a ?one size fits all? approach. Ideally, the corporate strategy for a large and diversified business serves as the umbrella strategy that provides structure, goals and measurement for the business unit strategic plans to link back to as an anchor. The business unit strategies exist to propel results for their organization that will satisfy the overall corporate strategic goals.

Strategy is all about people. It is about the people that the organization serves through the execution of its mission. Strategy is also about the people within the organization that must execute the strategy. Customers, employees, partners and suppliers are known and understood better at the business unit level where they are directly linked. As such, strategic planners in the corporate organization must develop overarching strategic plans that leave room for the nuances of tactical execution to remain the business unit management team?s responsibility.

Corporate-level strategic plans must cast the broad mission, vision and strategy in aggregate terms for the overall business, but it must also define strategic key outcomes that can be translated and measured at the business unit level (e.g. revenue growth, market share and profitability targets to name a few).? Of course, business units must have strategic plans as well. Business unit plans should map back to the broader strategic goals defined in the corporate strategic plan while also relating line-of-business level strategic goals, objectives and tactics that advance the business unit strategy.

The relationship between the corporate-level strategic plan and the individual business unit plans form a synchronized structure that pushes and pulls the organization in one direction. At the same time, this symbiotic structure leaves the accountability for leveraging intimate knowledge of customers, competitors, employees and culture to the business layer closest to the action. It allows the business unit the flexibility to plan autonomously while remaining aligned with the overall corporate strategy and goals. This is the strategic planning model mirrored in most corporate to subsidiary business relationships.

Maintaining alignment between corporate goals and business unit strategies in a bi-directional planning model is the key to making this structure and process work. A planning governance model that works up and down the business layers is essential to maintaining alignment and tracking execution. So is corporate performance management. Performance management helps tie the measurement of the business unit leader?s performance to the attainment of corporate goals as well as those defined in the business unit-level strategic plan. Performance management also applies to the employees within the business units, who are measured on goal attainment within the operating unit where they have direct impact on strategic plan execution.

In Summary

Bi-directional planning works well for diverse portfolio businesses. The level of detail involved in corporate strategic planning is not well-matched to that involved in business unit strategic planning. Implementation methods (execution tactics) are more effectively defined at the business unit level, as they are dependent on organization structure, culture and business knowledge. Another reason that a separation of planning layers is beneficial is because corporate strategists have a different perspective than their counterparts in the business units. Corporate strategists are more tuned into the aggregated set of competitors they face across the organization?s diversified lines of business, so strategic maneuvers at the parent company level tend to be broad. Conversely, business unit?s are more attuned to their specific competitors. With their more intimate knowledge of their landscape, business units can craft better strategies to address competitors and trends in their industry niche. Diversified planning, while it may seem more complicated on the surface, actually removes complexity by allowing each strategic plan to be written more concisely and measured more accurately.

Source: http://blog.vistage.com/business-strategy-and-management/integrating-business-unit-strategies-into-a-synchronized-corporate-strategic-plan/

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Where's the snow? Not in Lower 48, but elsewhere

[unable to retrieve full-text content]WASHINGTON (AP) ? Snow has been missing in action for much of the U.S. the last couple months. But it's not just snow. It's practically the season that's gone AWOL.

Source: http://hosted2.ap.org/APDEFAULT/b2f0ca3a594644ee9e50a8ec4ce2d6de/Article_2012-02-01-US-SCI-Where's-The-Snow?/id-6d9e9efe843345e6a8139a917dbfe06f

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Lose The Fat And Gain Muscle With These Easy Fitness Tips

Are you in good shape but want to get into better shape for a 5K or perhaps a marathon? If so, then consider the pieces of advice in the below article. These tips can help you get into the top notch shape in order to compete in your particular race.

Make sure that you are breathing deeply whenever you run. Your body needs lots of oxygen when you are working out so do your best to take long deep breaths that make your belly rise each time you inhale. This is also a great way to increase your lung capacity.

A great tip to help you get physically fit is to start playing racquetball. Playing racquetball can burn a lot of calories because you constantly have to run back and forth to hit the ball. Try to find a racquetball club near you that offers a fair amount of courts.

When you start your muscle building/strengthening program, start out by completing compound exercises that work several muscles groups at a time. Training large muscle groups will prepare you for more individualized exercises in the future and reduce your chances of injuries. Take it step by step and properly prepare your muscles.

To get you started and motivated in a new physical fitness program, keep a journal and track your exercise activity. This journal will boost your awareness and measure the accomplishments that you have made over time. Treat the task, of getting physically fit, as if it means that your life depends upon it.

Muscle injuries can bring us down, but getting back into exercising as soon as you can will help you heal quicker. Start out slowly, trying just a few minutes at a low intensity just to test yourself. As soon as you experience pain, stop. When you are finished apply ice for twenty minutes. Continue the next day trying to do a little more.

To develop great looking abdominal muscles make sure that, in addition to isolated ab work, you include heavy free weight compound exercises in your training. Basic old-school lifts like squats and dead-lifts are great exercises to build your abdominal muscles because during these lifts your abs are forced to contract in order to maintain proper posture.

Taking up a sport can be a great help to a personal fitness routine. A sport that requires some cardiovascular exertion and muscle strength will provide the benefits of exercise that every fitness program should include. A sport that you enjoy can be easier to participate in and stick with than simply working out for the sake of working out.

The best way to build up your forehand strength for use with sports like tennis and racquetball is to do exercises with a crumpled newspaper. To do this properly, lay the paper flat on a surface. Start at a corner and crumple it into a ball shape with your dominant hand for about 30 seconds. Do the exact same with the other hand.

Remember, you can work out no matter what your age, health, or fitness level. There is a fitness plan out there that can work for your life. The tips we went over here are just a starting point, get out there are learn all you can so that you can get the body that you want.

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elderly care,home care - Importance of Elderly Care Services

by Raman Maan
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elderly care,home care More Details about elderly care,home care here.

It is obvious that the Elderly Care is the excellent and unsurpassed option to care for the seniors. Everyone wants to keep their seniors always protected, happy and secure, but due to lack of time and busy schedule they are not able to do that. Elder care is like a care of a little child. They are not able to do the certain activities of their daily schedule. Dementia is a common disease that occurs in the people with the age and elders mostly suffer from this problem. So they require regular medical check-up and care. Aged people become weak and prone to fall at their old age. In such situation, you need to know the safety tips and instructions and to pursue them to frustrate mishaps and accidents.

The care of the aged is difficult for family members and due to such reason; most of the families go with the hiring of someone to care for their relatives. The most effective and inexpensive choice to care for your seniors or aged relatives and you can get services for your elderly parents or relatives without moving them from their usual place. You should make the accurate adjustment for your loved ones, if you want that their remaining days will spend well and fine.

If, you provide home care for your loved ones, especially when you are too far away, it can be something to respect what you have. The massive benefit in this is having your own loved ones taken care of your own home or in their home environment. There are several agencies and service organizations which help you in providing the best care and required services for your loved ones. They will provide guarantee for their work and trust. These provide basic need such as vigorous and healthy meals, medical services, washing, dressing the oldest and make sure that the work, cleaning, to be visited in their homes. In these organizations, the care provider is also there to provide personal care like staying with the elderly when the family is out the house; break care; financial management assisting on bill payments and medical services, etc. Home care occupies more than just checking on an older person. These bring a sense of security to our seniors that they are being taken care of in a known environment. They feel there like they are at their home. The care providers love them like a family member.

Author name is Ramandeep Kaur. She loves to share her views. Now she is sharing information on Elderly Care.

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Raman Maan

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More Details about elderly care,home care here.

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The Secret Circle Scoop: Diana's New Love, John Blackwell's Arrival and the Balcoin Child (omg!)

Who is John Blackwell's other child in The Secret Circle's coven?

While both audiences and cast wait with bated breath to find out, Circle's Shelley Hennig thinks the idea of good-girl Diana turning out to be the other Balcoin child would finally explain Diana's mysterious connection to Cassie (Britt Robertson). TVGuide.com pumped Hennig for scoop on who the other Balcoin child could be, as well as the arrival of Cassie's seemingly evil father, John Blackwell (Joe Lando). Plus: Sorry, Adam (Thomas Dekker), but Diana's getting a new love interest!

Exclusive: The Secret Circle casts Cassie's mysterious father John Blackwell

We got a glimpse of the tragedy from 16 years ago in last week's episode, but it's still unclear what happened to most of the circles' parents. Will we get more insight into that?
Shelley Hennig: What I love is that we were all hoping to know what happened 16 years ago and they do a flashback, yet now I have 10 more questions about what happened.? We learn a little more each episode. The circle is pretty savvy, so I think they're going to do whatever they can to figure that out. That's been their mission since Episode 1.

Because the circle is on the outs, how will they react when Cassie's father, John Blackwell, comes into the picture?
Hennig: The whole circle is trying to figure out what Blackwell's intentions are, and it will be interesting to see how each person in the circle reacts to it. Diana's actually the one who's looking at it like, "Oh my God, you've got your dad back! We've all wished we could have our dead parents back, and here you are." Diana's really intrigued with him being back, but also on guard, just like the rest of us are.

Will Cassie ever take her dark magic too far?
Hennig: Hasn't she gotten us in enough trouble? Goodness! [Laughs] I'm sure with finding out more information about dark magic, Blackwell and the witch hunters, things are still going to be taken too far. It's kind of what you have to do in order to find out more information.

The Secret Circle: Jake is back! Will the other Balcoin child be revealed?

Who do you think is the other Balcoin child?
Hennig: I think it'd be awesome [if Diana is] and I think it would explain the connection that Diana has with Cassie, and why she goes to Cassie when she breaks up with Adam. Like, who does that? Who goes to the girl that now has your boyfriend? I think it will be interesting whatever way that they choose.?

Have the actors learned who the other Balcoin child is yet?
Hennig: No. Still in the dark. Not a clue. Audiences sit there waiting to watch each episode air. We're like that with the script. We're like, 'OK, are we going to get answers now?' We're all totally into this, and we talk about it all the time. Everybody has their own ideas, and it's just fun to come up with the way we think it's going to unfold.

Executive producer Andrew Miller mentioned that Diana will have a love interest this season. Could he be the newly cast Australian actor Tim Phillipps?
Hennig: Yes. [His character is] really handsome, really sweet and hopefully he's going to sweep Diana off her feet, if not long term, at least to help Diana get through this heartbreak with Adam. But who knows, maybe this guy's exactly what Diana needs.

Even though she'll have her own love interest, how will she feel about Adam and Cassie getting closer, especially with Jake (Chris Zylka) also in the picture?
Hennig: Diana's never going to forget. Let's face it: Adam's the only boyfriend she's ever had.?It's going to be hard for Adam and Diana. Adam has a distraction, so it's a little easier for him, but he even knows how special Diana and Adam's relationship was. She's putting on a hard exterior so that Adam can move on, but how long can she do that? That's what you'll discover in the next episode that airs.

Exclusive: The Secret Circle lands two new bad boys

Will Diana ever figure out what her dad, Charles (Gale Harold), is up to? He's taken evil to a new level since the show returned.
Hennig: Yes. This is what I'm trying to figure out. This is all about your bloodline, right?? So is there more Diana in Charles or is there more Charles in Diana? That's what I hope they explore.?And obviously, I hope there's more Charles in Diana, because it will be way more interesting.?

Do you want to see Diana turn evil like her pops? And who do you think is the other Balcoin child? Hit the comments with your thoughts.

The Secret Circle airs Thursdays at 9/8c on The CW.

Related Articles on TVGuide.com

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Top Ten Myths About Fat Loss Diets | barcelona-1.com

Several folks do not have a physical difficulty that could occur. Other people are merely lazy. The great news is the fact that you?ll be able to lose weight with out exercise. All you have to do is track what you eat to retain and use a calorie calculator could be identified on numerous Internet web sites, calculate the calories you burn. Furthermore, it calculates the quantity of calories you burn each and every day on foot, walking, climbing stairs, gardening, cooking, etc. To shed weight you need to be sure that the calories are burned even more calories they consume. Right here are some tips on how to shed weight without physical exercise, will aid accomplish this aim.Numerous individuals don?t have a physical issue that might happen. Other individuals are simply lazy. The excellent news is that you are able to lose weight without having exercise. All you need to do is track what you eat to maintain and use a calorie calculator can be identified on several Internet web sites, calculate the calories you burn. Additionally, it calculates the amount of calories you burn each and every day on foot, walking, climbing stairs, gardening, cooking, and so on. To shed weight you need to make sure that the calories are burned even more calories they consume. Right here are some ideas on how to lose weight with out exercise, will help achieve this objective.

Actually, you can find numerous things that may be completed to get ideal body weight without doing the exercises how to lose weight quickly . You can do these items with small discipline to ensure that you?ll be able to make it as habit. You will find that the result for this technique either in brief time or in particular length of time. 1 factor for positive, these issues will assist you to get perfect weight efficiently. Spend attention towards the following issues and get your body weight ideal!

Discover beneath are the suggestions on the best way to lose weight without having exercise:

Get rid of water weight. Specific medicines like birth manage pills and prescribed steroids trigger bloating. Drink eight to ten glasses of water each and every day to get rid of excessive fluids out of one?s body and lose weight.

Decrease your calorie intake. To shed weight with out exercise, eat 500 less calories each and every day. Stay with this program and you?ll shed a minimum of 1 pound per week.

Eat Fiber and Proteins: If you?re looking for appetite suppressants to lose weight quick without having workout, or preserve a wholesome weight, then you have to have a fiber wealthy diet plan. Fiber leads to a sense of bounties with out additional intake of calorie. Within the exact same way, proteins supply power but don?t result in weight acquire. Fish, lean meats, egg white, pulses, legumes, contain great quantity of proteins.

No Sweets and Fat: Although you adore to overeat on your preferred desserts, they include large levels of calories and result in a fast weight acquire. Comparable will probably be the case with fats. Consequently, inside your weight reduction without having workout method, you have to completely reduce these foods. If searching for option of specifically the same, you might be able to have fruits with yogurt for dessert; and pick low-fat variants of milk to reduce on fat.

When it comes to losing weight with out workout, sauna could be an additional thing that you simply should think about rather than paying attention towards the food and drink that you consume. Although you might be sweating within the sauna, you?ll burn some pounds of the body weight loss . You?ll find that it?s a very efficient because you only need to sit within the sauna and you will really feel the result in a short time. For the info, this ought to be carried out within the correct way given that it can be dangerous for you. Be sure that you might be hydrated once you are doing this approach. For secure, you ought to talk and talk about it with your doctor.

Make fat loss shakes. Use fruits or vegetables of your choice and voila! Drink or eat fruit chunks so that you can shed extra pounds.

Drink green tea or slimming tea. Take a cue from Chinese and get back in shape by drinking slimming tea to burn calories and have a glowing skin too.

NO a lot more junk food within your diet regime. Stop eating French fries or purchasing chips each and every time you pay a pay a visit to towards the supermarket.

Include low fat low sugar food items in your diet plan. You get a lot of variety of food items inside the industry which might be low in fat and deserts that are sugar totally free. Stick to them and see the weight melting off your body.

If you nonetheless feel hungry, snack on one thing wholesome like mangoes, apples or oranges rather than chips. Fruits also help you satisfy your sweet tooth.

Hope above suggestions will reveal quesion on the best way to shed weight without having physical exercise. Keep in mind that attempting to adhere to plenty of new rules may be the reason why lose weight so difficult for many individuals. You do not want to employ each and every program that people may well suggest. Choose the one which you in a position to complete and practice. The extremely very best element to total could be to attempt a couple of after which stick making use of the ones that show you the really best outcomes.

Source: http://www.barcelona-1.com/2012/02/02/top-ten-myths-about-fat-loss-diets/

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